BD mouse monoclonal PINCH 1 antibody 612710 was validated in knockout mice
DOI
http://dx.doi.org/10.13070/ko.en.6.1585
Date
2016-08-30

This is a knockout-validated antibody summary, based on the publication "Role of PINCH and its partner tumor suppressor Rsu-1 in regulating liver size and tumorigenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Company: BD

Antibody: PINCH 1

Catalog number: 612710

Summary

Mouse IgG2a monoclonal antibody raised against human PINCH peptide (aa. 120-220). It reacts to human, mouse, rat, and dog. It has been successfully used for western blot.

BD mouse monoclonal PINCH 1 antibody 612710 was validated in knockout mice figure 1
Figure 1. Western blot of PINCH 1 on hepatocytes isolated from PINCH1 and PINCH 2 double knockout mice and control mice. Actin is used as a load control. from Figure 2 [1].
Validation Method

WB, western blot

Sample

Hepatocytes from PINCH 1 and PINCH 2 double knockout (PINCH DKO) mice. Total protein was isolated from the mouse hepatocytes or whole livers of PINCH DKO and control mice using 1% sodium dodecyl sulfate (SDS) in RIPA buffer (20 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5% NP-40, 1% TX-100, 0.25% Sodium Deoxycholate (DOC), 0.6-2 µg/ml aprotinin, 10µM Leupeptin, 1µM Pepstatin).

Primary incubation

Tris-buffered saline Tween 20 containing 5% nonfat milk

Secondary incubation

Tris-buffered saline Tween 20 containing 5% nonfat milk

Detection

SuperSignal West Pico chemiluminescence substrate (Pierce, Rockford, IL) and exposed to a X-ray film (Lab Product, Sales, Rochester, NY).

References
  1. Donthamsetty S, Bhave V, Mars W, Bowen W, Orr A, Haynes M, et al. Role of PINCH and its partner tumor suppressor Rsu-1 in regulating liver size and tumorigenesis. PLoS ONE. 2013;8:e74625 pubmed publisher