BAP1 antibody | knockout validation | Santa Cruz Biotechnology sc-28383

DOI

//dx.doi.org/10.13070/ko.en.7.2254

Date

2017-04-08

This is a knockout-validated antibody summary, based on the publication "Loss of BAP1 function leads to EZH2-dependent transformation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz Biotechnology

Antibody: BAP1

Catalog number: sc-28383

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 430-729 of BAP1 of human origin. Recommended for detection of BAP1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

Bone marrow from control and Bap1 knockout mice. Cells were lysed in 150 mM NaCl, 20 mM Tris (pH 7.4), 5 mM EDTA, 1% Triton, protease arrest (EMD) and phosphatase inhibitors (Calbiochem).

Primary incubation

1:1,000 dilution.

Figure

Verification of Bap1 deletion by Western blot of control (littermate Bap1f/f mice, CON) and Bap1 knockout (Mx1-Cre Bap1f/f mice, Bap1 KO) bone marrow. Please see Figure 1a in the article [1].

References

Lafave L, Beguelin W, Koche R, Teater M, Spitzer B, Chramiec A, et al. Loss of BAP1 function leads to EZH2-dependent transformation. Nat Med. 2015;21:1344-9 pubmed publisher