This is a knockout-validated antibody summary, based on the publication "Loss of BAP1 function leads to EZH2-dependent transformation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz Biotechnology
Antibody: BAP1
Catalog number: sc-28383
Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 430-729 of BAP1 of human origin. Recommended for detection of BAP1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.
Western blot
Bone marrow from control and Bap1 knockout mice. Cells were lysed in 150 mM NaCl, 20 mM Tris (pH 7.4), 5 mM EDTA, 1% Triton, protease arrest (EMD) and phosphatase inhibitors (Calbiochem).
1:1,000 dilution.
Verification of Bap1 deletion by Western blot of control (littermate Bap1f/f mice, CON) and Bap1 knockout (Mx1-Cre Bap1f/f mice, Bap1 KO) bone marrow. Please see Figure 1a in the article [1].