BAP1 antibody | knockout validation | Cell Signaling 13271

This is a knockout-validated antibody summary, based on the publications "Intrinsic apoptosis shapes the tumor spectrum linked to inactivation of the deubiquitinase BAP1", as cited below [1], and "BAP1 is a haploinsufficient tumor suppressor linking chronic pancreatitis to pancreatic cancer in mice" for immunohistochemistry knockout validation (figure s2c) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: BAP1

Catalog number: 13271

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Leu709 within the BRCA1 interaction region of human BAP1 protein. Reacts with human, mouse and rat. Suitable for western blot and immunoprecipitation.

Validation Method

Immunocytochemistry

Sample

Control and Bap1-KO ES cells.

Detection

Western blot images were acquired with an Amersham Imager 600 (GE Health).

Figure

Western blots of the indicated proteins in ES cells cultured with (A) and without (B) emricasan. Please see Figure 3a in the article [1].

References
  1. He M, Chaurushiya M, Webster J, Kummerfeld S, Reja R, Chaudhuri S, et al. Intrinsic apoptosis shapes the tumor spectrum linked to inactivation of the deubiquitinase BAP1. Science. 2019;364:283-285 pubmed publisher
  2. Perkail S, Andricovich J, Kai Y, Tzatsos A. BAP1 is a haploinsufficient tumor suppressor linking chronic pancreatitis to pancreatic cancer in mice. Nat Commun. 2020;11:3018 pubmed publisher