This is a knockout-validated antibody summary, based on two publication "Mito-priming as a method to engineer Bcl-2 addiction" (Figure 1, discussed below) [1], and "Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency" (See supplement figure 1i in [2] ). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of BAK and BAX expression in CRISPR/CAS9 genome edited cell lines. Actin was probed as a loading control. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: BAK

Catalog number: 12105

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Gly75 of human Bak protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry and flow cytometry.

Western blot

Control, BAX-KO, BAK-KO and BAX/BAK-DKO SVEC cells stably expressing tBID-, BIM- or PUMA 2A GFP-BCL-xL.

1:1,000 dilution.