This is a knockout-validated antibody summary, based on the publication "β-Secretase BACE1 Is Required for Normal Cochlear Function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Abcam

Antibody: BACE1

Catalog number: ab108394

Summary: Rabbit monoclonal IgG against a sSynthetic peptide within human BACE1. Reacts with mouse, rat and human. Suitable for western blot and immunoprecipitation. Not recommended for immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) or flow cytometry.

Immunohistochemistry

Wild-type and BACE1−/− mice cochlea. Tissues were formaldehyde-fixed and processed to cryo-sections.

Buffer containing 10% normal goat serum (NGS), 0.3% Triton X-100, 20 mm phosphate buffer (PB), and 450 mm NaCl, for 1 h at room temperature.

1:50 dilution in blocking solution overnight at 4°C

Species-appropriate secondary antibodies coupled to Alexa Fluor dyes (Thermo Scientific and Abcam) for 90 min at room temperature.

Confocal imaging was performed with an upright LSM 710 Axio Examiner Z1 microscope using W-Plan-Apochromat 63×/1.0 M27 water-immersion objective (Carl Zeiss).

Expression of BACE1 was analyzed with immunohistochemistry on (A,B,E) cochlear cryosections. A, We detected strong anti-BACE1 immunosignals in cell bodies of SGN in BACE1+/+ mice that (B) were strongly reduced in BACE1-/-mice. The panels (A+B) show representative transmitted light (left) and corresponding confocal images (right) of cryo-sections through the spiral ganglion stained with primary antibodies directed against BACE1 (green). Please see Figure 2a,b in the article [1].