Atg9A antibody | knockout validation

Atg9A antibody | knockout validation | Cell Signaling 13509

This is a knockout-validated antibody summary, based on the publication "The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Atg9A

Catalog number: 13509

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Gly780 of human Atg9A protein. Reacts with human, mouse, rat and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Atg9a+/+ cells and Atg9a-/- MEF cells.

Detection

ECL western blotting detection reagents (Advansta, E-1119–50).

Figure

Immunoblot of ATG9A in Atg9a+/+ cells and Atg9a-/- cells under full medium conditions. Please see Figure 5h in the article [1].

References

Park J, Jung C, Seo M, Otto N, Grunwald D, Kim K, et al. The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14. Autophagy. 2016;12:547-64 pubmed publisher

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