Atg16L antibody | knockout validation | MBL international M150-3

This is a knockout-validated antibody summary, based on the publication "Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Atg16L antibody | knockout validation | MBL international M150-3 figure 1

Figure 1. Immunoblots confirming deletion of autophagy genes in BMDMs of indicated genotypes using RNP electroporation. NTC = non targeting control gRNA. From [1].

Antibody information

Mouse monoclonal IgG1,ĸ

Company: MBL international

Antibody: Atg16L

Catalog number: M150-3

Summary: Mouse monoclonal IgG1,ĸ against recombinant human ATG16L1 TV2 (85-588 a.a.). Reacts with human, mouse and rat. Suitable for western blot.

Validation Method

Western blot

Sample

Control and ATG16L1-KO BMDMs. Cell pellets were lysed in RIPA buffer +protease and phosphatase inhibitors (Roche).

Secondary incubation

Anti-mouse IgG-HRP (Cell Signaling Technology),

Detection

Enhanced chemiluminescence (western lightning-plus ECL, Perkin Elmer).

References

Lim J, Park H, Heisler J, Maculins T, Roose Girma M, Xu M, et al. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. elife. 2019;8: pubmed publisher

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