Atg14 antibody | knockout validation | MBL International M184-3

This is a knockout-validated antibody summary, based on the publication "Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Atg14 antibody | knockout validation | MBL International M184-3 figure 1

Figure 1. Western blot analysis of Atg14 in MCF7.control and MCF7.beclin 1 cells transduced with a CRISPR/Cas9 lentivirus containing either no sgRNA (Empty) or two sgRNAs targeting the indicated gene. From [1].

Antibody information

Mouse monoclonal IgG2a ĸ

Company: MBL International

Antibody: Atg14

Catalog number: M184-3

Summary: Mouse monoclonal antibody against human Atg14 (167-404 aa). Reacts with human by western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and Atg14 KO MCF7 cells.

Primary incubation

1:500 dilution.

References

Wijshake T, Zou Z, Chen B, Zhong L, Xiao G, Xie Y, et al. Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher

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