Atg13 antibody | knockout validation | Cell Signaling 13468

This is a knockout-validated antibody summary, based on the publication "Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Atg13 antibody | knockout validation | Cell Signaling 13468 figure 1
Figure 1. Western blot analysis of Atg13 in MCF7.control and MCF7.beclin 1 cells transduced with a CRISPR/Cas9 lentivirus containing either no sgRNA (Empty) or two sgRNAs targeting the indicated gene. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Atg13

Catalog number: 13468

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Asn230 of human Atg13 protein. Reacts with human by western blot, immunoprecipitation and immunofluorescence (immunocytochemistry).

Validation Method

Western blot

Sample

Control and Atg13 KO MCF7 cells.

Primary incubation

1:500 dilution.

References
  1. Wijshake T, Zou Z, Chen B, Zhong L, Xiao G, Xie Y, et al. Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher