This is a knockout-validated antibody summary, based on the publication "CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot validation of CRISPR-Cas9 generated knockout cell lines that were pre-validated by deep sequencing to bear all-allelic frame shift mutations (knockout (KO) A, B) or heterozygous mutations (−/+). Size marker bands are indicated in the first column; expected protein sizes are given in the last column. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Artemis

Catalog number: 13381

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Pro367 of human artemis protein. Reacts with human and monkey by western blot and immunoprecipitation.

Western blot

WT and Artemis-KO HEK 293 cells. Cells were lysed in 50 ul of Laemmli buffer per 96-well.

5% milk.

1:1,000 dilution.

NHEJ1 XLF rabbit Ab (Cell Signaling 2854); DNA-PK rabbit Ab (Cell Signaling 4602); and ATM 2C1 mouse mAb (Abcam ab78) are also KO validated.

The same clone (2C1) is sold as Novus Biologicals NB100-309; Santa Cruz Biotechnology sc-23921; LifeSpan Biosciences LS-C20116, LS-B1683; Abnova MAB12913; Abcam ab78; Invitrogen MA1-23152; GeneTex GTX70103.