Argonaute-2 antibody | knockout validation | Sino Biologicals 50683-R036

This is a knockout-validated antibody summary, based on the publication "AGO2 promotes tumor progression in KRAS-driven mouse models of non-small cell lung cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Argonaute-2 antibody | knockout validation | Sino Biologicals 50683-R036 figure 1
Figure 1. Immunoblot analysis of KPC-Ago2−/− organoid-derived cells transfected with constructs encoding wild-type AGO2 (WT AGO2), AGO2K112A/E114A (K112A/E114A), and AGO2K98A (K98A). From [1].
Antibody information

Rabbit monoclonal IgG

Company: Sino Biologicals

Antibody: Argonaute-2

Catalog number: 50683-R036

Summary: Rabbit monoclonal antibody against recombinant mouse AGO2 protein. Reacts with mouse. Suitable for immunoprecipitation, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot | Immunohistochemistry

Sample

WT and AGO2-KO lung nodules-derived organoids (WB) and lung tissues (IHC).

Blocking agent

IHC: 3% hydrogen peroxide (Sigma).

Primary incubation

WB: 1:1,000 dilution.

IHC: 1:100 dilution in 10% normal goat serum (NGS) (in PBS).

Secondary incubation

IHC: species-specific VECTSTAIN Elite ABC kits (Vector Labs).

Detection

IHC: DAB Peroxidase Substrate kit (Vector Labs).

Clone note

The same clone (036) is sold as Invitrogen MA5-31031; Sino Biological 50683-R036.

References
  1. Tien J, Chugh S, Goodrum A, Cheng Y, Mannan R, Zhang Y, et al. AGO2 promotes tumor progression in KRAS-driven mouse models of non-small cell lung cancer. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher