This is a knockout-validated antibody summary, based on the publication "Arginase 2 Deficiency Promotes Neuroinflammation and Pain Behaviors Following Nerve Injury in Mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative western blots and corresponding quantitative data for band intensity, comparing the expression of Arg2 in the spinal dorsal horn of mice on day 7 from the sham WT (n = 4), the sham Arg2 KO (n = 4), the SNT WT (n = 4), and the SNT Arg2 KO (n = 4) mice. Arg2 was expressed more strongly in the SNT WT mice, compared to the sham WT mice. From [1].

Mouse monoclonal IgA (kappa light chain)

Company: Santa Cruz

Antibody: Arg2

Catalog number: sc-393496

Summary: Mouse monoclonal IgA (kappa light chain) specific for an epitope mapping between amino acids 304-335 near the C-terminus of human Arg2. Recommended for detection of Arg2 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including and canine, bovine and porcine.

Western blot

WT and Arg2-KO mouse spinal dorsal horn.

5X Phospho-BLOCK Solution (Translab, Daejeon, Korea, TLP-115.1P) diluted to 1X in distilled water.

1:50 dilution overnight.

Enhanced chemiluminescence detection system (Habersham, Little Chalfont, UK).