This is a knockout-validated antibody summary, based on the publication "Aquaporin 1 affects pregnancy outcome and regulates aquaporin 8 and 9 expressions in the placenta", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. AQP1 knockout mice exhibit a decrease of AQP9 protein in placenta and foetal membrane and an increase of AQP8 protein in foetal membrane. Expression of AQP1, AQP3, AQP8 and AQP9 proteins was measured by western blotting in placenta at 13.5 gestational days. From [1].

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: Aquaporin 1/AQP1

Catalog number: sc-25287

Summary: Mouse monoclonal antibody against amino acids 215-269 of human AQP1. Recommended for detection of AQP1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

Control and AQP1-KO mouse placenta and foetal membrane.

5% skimmed milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T, pH 7.4) at room temperature for 2 h.

1:1,000 dilution at 4 °C overnight.

1:5,000 dilution horseradish-peroxidase-conjugated secondary antibody (Biosharp, China) for 2 h at room temperature.

Enhanced chemiluminescence (ECL) substrate (Thermo, USA). Iimages were captured with an Amersham Imager 600 system (General Electric Company, USA).