This is a knockout-validated antibody summary, based on the publication "Diminished OPA1 expression and impaired mitochondrial morphology and homeostasis in Aprataxin-deficient cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis showing complete absence of APTX in CRISPR-mediated APTX knockout (KO) U2OS cells (lane 2) and in cells prepared from AOA1 patients. From [1].

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: Aprataxin

Catalog number: sc-374108

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 57-356 mapping at the C-terminus of human Aprataxin. Recommended for detection of Aprataxin isoforms 1-10 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

Control and APTX-KO U2OS cells.