Apolipoprotein CI antibody | knockout validation | Abcam ab198288

This is a knockout-validated antibody summary, based on the publication "Characterization of apolipoprotein C1 in hepatitis C virus infection and morphogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: Apolipoprotein CI

Catalog number: ab198288

Summary: Rabbit monoclonal IgG against a recombinant full length protein within human Apolipoprotein CI (aa 1 to the C-terminus). Reacts with human. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence, immunoprecipitation and flow cytometry.

Validation Method

Western blot

Sample

Parental and ApoC1-/- Huh-7.5 cells.

Blocking agent

5% nonfat milk.

Secondary incubation

HRP-conjugated anti-rabbit secondary antibody.

Detection

Enhanced chemiluminescence (ECL) kit (Pierce).

Figure

Determination of apolipoproteins expression in parental and apoC1-/- − 13 cell lines by western blot. Please see Figure 7a in the article [1].

References
  1. Zhang H, Qiao L, Luo G. Characterization of apolipoprotein C1 in hepatitis C virus infection and morphogenesis. Virology. 2018;524:1-9 pubmed publisher