Apg3 antibody | knockout validation | Abcam ab108251

This is a knockout-validated antibody summary, based on the publication "Oncolytic Reovirus Infection Is Facilitated by the Autophagic Machinery", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Apg3 antibody | knockout validation | Abcam ab108251 figure 1
Figure 1. Western blot of Atg3 and actin (loading control) in Atg3wt, Atg3−/−, and Atg3+/+ MEFs infected with R124 at an MOI of 0.1 or 10. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: Apg3

Catalog number: ab108251

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human Apg3 (Atg3) (N terminal). Reacts with mouse, rat and human. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence and flow cytometry. Unsuitable for immunoprecipitation.

Validation Method

Western blot

Sample

Atg3wt, Atg3−/−, and Atg3+/+ MEFs. Protein lysates were prepared in Giordano lysis buffer (50 mM Tris·HCl pH 7.4, 250 mM sodium chloride, 0.1% Triton X-100, and 5 mM EDTA) supplemented with protease inhibitors (Complete mini tablets, Roche Diagnostics, Almere, The Netherlands).

References
  1. Kemp V, Dautzenberg I, Limpens R, van den Wollenberg D, Hoeben R. Oncolytic Reovirus Infection Is Facilitated by the Autophagic Machinery.. Viruses. 2017;9: pubmed publisher