This is a knockout-validated antibody summary, based on two publications "Heterotetrameric annexin A2/S100A10 (A2t) is essential for oncogenic human papillomavirus trafficking and capsid disassembly, and protects virions from lysosomal degradation" (western blot, Figure 1a in the article) [1], and "Annexin 2-CXCL12 interactions regulate metastatic cell targeting and growth in the bone marrow" (discussed below) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: Annexin II

Catalog number: 610069

Summary: Mouse monoclonal IgG1 against human Annexin II aa. 123-328. Reacts with human, mouse, rat, dog, and chicken. Suitable for western blot, immunoprecipitation, immunofluorescence, immunohistochemistry-formalin (antigen retrieval required).

Immunocytochemistry | Immunohistochemistry

Bone marrow stromal cells and bone sections from wild-type and Anxa2 knocked-out mice. IC: Cells were fixed with 10% neutral buffered formalin (cat. HT501320, Sigma) and permeabilized with PBT (1:500 dilution of Triton-X100 in PBS). IHC: long bones were embedded in paraffin for sectioning.

IHC: Image-iT FX signal enhancer (cat. I36933, Life Technology, Madison, WI) for 30 min.

1:25 dilution for 2 hours at room temperature.

Primary antibodies were combined with reagents of Zenon Alexa Fluor 488 (green) or 555 (red) labeling kit (Life Technology).

Olympus FV-500 confocal microscope.

Please see Figure 1a,b in the article [2].