Annexin A1 antibody | knockout validation | Invitrogen (ThermoFisher Scientific) 71-3400
DOI
//dx.doi.org/10.13070/ko.en.7.1932
Date
2017-01-13

This is a knockout-validated antibody summary, based on the publication "Annexin A1 contributes to pancreatic cancer cell phenotype, behaviour and metastatic potential independently of Formyl Peptide Receptor pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Annexin A1 antibody | knockout validation | Invitrogen (ThermoFisher Scientific) 71-3400 figure 1
Figure 1. From Figure 1 in [1]. Western blot of lysates of wild-type and annexin-A1 knockout MIA CaPa-2 cells probed with anti-annexin A1 antibody. Tubulin serves as a loading control.
Antibody information

Rabbit polyclonal

Company: Invitrogen (ThermoFisher Scientific)

Antibody: Annexin A1

Catalog number: 71-3400

Summary: Rabbit polyclonal antibody raised against yeast-expressed full length human annexin A1.

Supplier recommended for WB, IP, ICC, IHC, flow cytometry and elisa.

Reacts with human, mouse and rat annexin A1.

Annexin A1 antibody | knockout validation | Invitrogen (ThermoFisher Scientific) 71-3400 figure 2
Figure 2. From Figure 2 in [1]. Wild-type and annexin-A1 knockout MIA PaCa-2 cells were stained with anti-annexin-A1 antibody (red).
Validation Method

Western blot

Immunocytochemistry

Sample

Western blot: lysates of wild-type and annexin A1 knockout MIA PaCa-2 cells.

Immunocytochemistry: paraformaldehyde-fixed wild-type and annexin A1 knockout MIA PaCa-2 cells.

Blocking agent

Western blot: not stated.

Immunocytochemistry: PBS containing 20% goat serum.

Primary incubation

Western blot: anti-annexin A1 antibody diluted 1:10000.

Immunocytochemistry: anti-annexin A1 antibodt diluted 1:100.

Secondary incubation

Western blot: not stated.

Immunocytochemistry: AlexaFluor-conjugated anti-rabbit IgG (1:1000; Molecular Probes).

Detection

Western blot: ECL reagent (GE Healthcare).

Immunocytochemistry: Zeiss confocal microscope with Zeiss ZEN confocal software/Image J.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Belvedere R, Bizzarro V, Forte G, Dal Piaz F, Parente L, Petrella A. Annexin A1 contributes to pancreatic cancer cell phenotype, behaviour and metastatic potential independently of Formyl Peptide Receptor pathway. Sci Rep. 2016;6:29660 pubmed publisher