This is a knockout-validated antibody summary, based on the publication "Defining new mechanistic roles for αII spectrin in cardiac function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2b, κ

Company: BioLegend

Antibody: Alpha-II Spectrin

Catalog number: 803201

Summary: Mouse monoclonal IgG2b, κ against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. Reacts with human, mouse, rat, and Drosophila. Suitable for western blot, immunocytochemistry and Immunohistochemistry (paraffin).

Western blot | Immunocytochemistry

Ventricular myocytes from II spectrin cKO and control mice.

WB: overnight at 4 °C.

IF: primary antibody diluted in fish gelatin buffer overnight at 4 °C

WB: Donkey anti-mouse (Jackson ImmunoResearch Laboratories, Bio-Rad).

IF: secondary antibodies (donkey anti-mouse 488) in fish gelatin buffer for 4 h at room temperature.

IF: Images were acquired at room temperature using an LSM 780 AxioObserver microscope (Zeiss) (40 x 1.30 NA lens, pinhole = 1.0 airy disc) and analyzed using ZEN imaging software (Zeiss).

Figure: Immunoblots for II spectrin normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in isolated adult ventricular myocytes from II spectrin cKO and control mice. Results demonstrate (B–D) reduction of full-length (250 kDa) and degraded (150 kDa) II spectrin from isolated adult ventricular myocytes from II spectrin cKO mice compared with control mice. Staining of II spectrin (red) and desmin (green) in adult cardiomyocytes isolated from (H) control and (J) II spectrin cKO mice. I, magnified image of control myocytes demonstrates localization of II spectrin at lateral membrane (white arrows), Z-lines (white arrowheads), and intercalated disc (yellow arrowheads). Scale bars 20 m. Please see Figure 2b,c,h,j in the article [1].

The same clone (D8B7) is sold as BioLegend 803201, 803205, 803206, 803207, 803208, 803209, 803210, 803211, 803212; Abcam ab11755; Invitrogen MA1-91103.