Alix antibody | knockout validation

Alix antibody | knockout validation | BioLegend 634502

This is a knockout-validated antibody summary, based on the publication "ATG9A protects the plasma membrane from programmed and incidental permeabilization", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Alix antibody | knockout validation | BioLegend 634502 figure 1

Figure 1. Confirmation by immunoblotting of ALIX and TSG101 knockdown as well as CRISPR–Cas9 KO of ALIX in HeLa cells. From [1].

Antibody information

Mouse monoclonal IgG1, κ

Company: BioLegend

Antibody: Alix

Catalog number: 634502

Summary: Mouse monoclonal antibody against recombinant full-length human Alix protein. Reacts with human, mouse, and frog. Suitable for western blot, immunoprecipitation (reported) and immunocytochemistry (reported).

Validation Method

Western blot

Sample

WT and ALIX KO HeLa cells.

Primary incubation

1:1,000 dilution.

Clone note

The same clone (3A9) is sold as Bio-Rad MCA2493; BioLegend 634501, 634502, 634504; Novus Biologicals NB100-65678; OriGene TA328026, AM01318PU-N, TA352527; Santa Cruz Biotechnology sc-53538; LifeSpan Biosciences LS-C41064, LS-C44918; Abcam ab117600; Invitrogen MA1-83977; Cell Signaling Technology 2171.

References

Claude Taupin A, Jia J, Bhujabal Z, Garfa Traoré M, Kumar S, da Silva G, et al. ATG9A protects the plasma membrane from programmed and incidental permeabilization. Nat Cell Biol. 2021;23:846-858 pubmed publisher

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