Aldolase + Aldolase B + Aldolase C antibody | knockout validation

Aldolase + Aldolase B + Aldolase C antibody | knockout validation | Abcam ab169544

This is a knockout-validated antibody summary, based on the publication "Loss-of-Function Genetic Screening Identifies Aldolase A as an Essential Driver for Liver Cancer Cell Growth Under Hypoxia", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Stable ALDOA knockout in HCCs by CRISPR/Cas9 lentiviral sgRNAs. The knockout effect was verified at protein levels by Western blot. From [1].

Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: Aldolase + Aldolase B + Aldolase C

Catalog number: ab169544

Summary: Rabbit monoclonal antibody against a synthetic peptide within human Aldolase (aa 1-100). Reacts with mouse, rat, human, and pig. Suitable for western blot and flow cytometry (intra). Unsuitable for immunocytochemistry/immunofluorescence or immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

WT and ALDOA-KO SMMC-7721 and HepG2 cell lines.

Primary incubation

1:1,000 dilution 4 °C overnight.

Secondary incubation

At room temperature for 1h.

Detection

Chemiluminescence visualizer (4600, Tanon, China).

References

Niu Y, Lin Z, Wan A, Sun L, Yan S, Liang H, et al. Loss-of-function genetic screening identifies ALDOA as an essential driver for liver cancer cell growth under hypoxia. Hepatology. 2021;: pubmed publisher

contribute

If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.