Akt2 antibody | knockout validation | Cell Signaling 5239

This is a knockout-validated antibody summary, based on the publication "Rapid interrogation of cancer cell of origin through CRISPR editing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Akt2 antibody | knockout validation | Cell Signaling 5239 figure 1
Figure 1. Western blot showing codisruption of AKT1 and AKT2 proteins in a patient-derived prostate organoid line by cRNP. From [1].
Antibody information

Mouse monoclonal IgG1

Company: Cell Signaling

Antibody: Akt2

Catalog number: 5239

Summary: Mouse monoclonal antibody against a synthetic peptide surrounding Leu110 of human Akt2. Reacts with human, mouse, rat and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and AKT2-KO patient-derived prostate organoids.

Primary incubation

1:1,000 dilution overnight at 4 °C.

Secondary incubation

HRP-conjugated antibodies.

Detection

Chemiluminescence.

References
  1. Feng W, Cao Z, Lim P, Zhao H, Luo H, Mao N, et al. Rapid interrogation of cancer cell of origin through CRISPR editing. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher