This is a knockout-validated antibody summary, based on publications "AKT isoform-specific expression and activation across cancer lineages", as cited below [1], "AKT1 Regulates Endoplasmic Reticulum Stress and Mediates the Adaptive Response of Pancreatic β Cells" for western blot knockout validation (figure 5a,6b) [2], "Immunohistological Examination of AKT Isoforms in the Brain: Cell-Type Specificity That May Underlie AKT's Role in Complex Brain Disorders and Neurological Disease" for western blot knockout validation (figure 7a) [3], and "AKT Ser/Thr kinase increases V-ATPase-dependent lysosomal acidification in response to amino acid starvation in mammalian cells" for western blot knockout validation (figure 5a) [4]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of AKT1 in HCT116 parental, AKT2−/−, and AKT1−/− cells serum starved overnight and treated (or not treated) with insulin (20 μg/ml) for 30 min. ERK immunoblotting showed equivalent loading. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Akt1

Catalog number: 2938

Summary: Rabbit monoclonal IgG against a synthetic peptide surrounding Leu110 of human Akt1. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Western blot

HCT116 parental, AKT2−/−, and AKT1−/− cells. Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, and 0.1% sodium dodecyl sulfate), supplemented with protease inhibitor and phosphatase inhibitor cocktails (Pierce Biotechnology, Rockford, IL).