Ah Receptor antibody | knockout validation | Santa Cruz sc-74571

This is a knockout-validated antibody summary, based on the publication "Aryl Hydrocarbon Receptor Defect Attenuates Mitogen-Activated Signaling through Leucine-Rich Repeats and Immunoglobulin-like Domains 1 (LRIG1)-Dependent EGFR Degradation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Ah Receptor antibody | knockout validation | Santa Cruz sc-74571 figure 1
Figure 1. AHR was undetectable in samples obtained from AHR-KO (C57BL/6−Ahrtm1.2Arte) mice. From [1].
Antibody information

Mouse monoclonal IgG2a κ

Company: Santa Cruz

Antibody: Ah Receptor

Catalog number: sc-74571

Summary: Mouse monoclonal antibody against amino acids 637-848 of human Ah Receptor. Recommended for detection of Ah Receptor of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Immunohistochemistry

Sample

WT and AHR-KO mouse lungs.

Detection

Peroxidase-labeled specimens were observed on a Nikon light microscope equipped with a Polychrome-III camera (YC technology, New Taipei City, Taiwan) and Image Eye software (FMJ Software, Stockholm, Sweden).

References
  1. Hsu H, Chen H, Tsai C, Liao P, Chan Y, Lee Y, et al. Aryl Hydrocarbon Receptor Defect Attenuates Mitogen-Activated Signaling through Leucine-Rich Repeats and Immunoglobulin-like Domains 1 (LRIG1)-Dependent EGFR Degradation. Int J Mol Sci. 2021;22: pubmed publisher