Ah Receptor antibody | knockout validation

Ah Receptor antibody | knockout validation | Santa Cruz sc-101104

This is a knockout-validated antibody summary, based on the publication "Targeting the Aryl Hydrocarbon Receptor With Indole-3-Aldehyde Protects From Vulvovaginal Candidiasis via the IL-22-IL-18 Cross-Talk", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Ah Receptor antibody | knockout validation | Santa Cruz sc-101104 figure 1

Figure 1. AhR protein expression by immunofluorescence staining of vaginal sections (Scale bars, 100 μm) in infected mice treated with 3-IAld. From [1].

Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: Ah Receptor

Catalog number: sc-101104

Summary: Mouse monoclonal IgG1 (kappa light chain) against recombinant human Ah Receptor. Recommended for detection of Ah Receptor of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Immunocytochemistry

Sample

WT and Ahr−/− mouse vagina. Tissues were removed and fixed in 10% phosphate-buffered formalin, embedded in paraffin and sectioned at 5 μm.

Primary incubation

Overnight at 4°C.

Detection

Images were acquired with a 40× objective and the analySIS image processing software.

References

Borghi M, Pariano M, Solito V, Puccetti M, Bellet M, Stincardini C, et al. Targeting the Aryl Hydrocarbon Receptor With Indole-3-Aldehyde Protects From Vulvovaginal Candidiasis via the IL-22-IL-18 Cross-Talk. Front Immunol. 2019;10:2364 pubmed publisher

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