This is a knockout-validated antibody summary, based on the publication "Whole animal knockout of smooth muscle alpha-actin does not alter excisional wound healing or the fibroblast-to-myofibroblast transition", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b
Company: Sigma-Aldrich
Antibody: Actin, Smooth Muscle
Catalog number: A7607
Summary: Mouse monoclonal IgG2b against a partially purified chicken gizzard actin. Reacts with chicken, monkey, human, rat, and mouse. Suitable for western blot, immunocytochemistry, immunohistochemistry and ELISA.
Western blot
WT and SMαA-null (SMαA−/−) primary dermal fibroblasts from mice cultured for 4 days in complete medium with 1 ng/ml TGF-β1 (Sigma-Aldrich) to promote myofibroblast differentiation. WB: Whole cell lysates were collected in 1% SDS lysis buffer.
WB: Blocking Buffer (Rockland Immunochemicals, Inc., Gilbertsville, PA).
WB: 1:300 dilution dilution in Blocking Buffer overnight at 4°C.
WB: ECL Western Blotting Substrate (Pierce, Rockford, IL) and a BioSpectrum 600 Imaging System (UVP, Upland, CA).
Immunoblot of WT and SMαA-null (SMαA−/−) fibroblasts with clone CGA7 antibody to determine SMγA and SMαA levels. Arrow indicates molecular weight of actin. Fibroblasts were cultured with TGF-β1 for 4 days to promote myofibroblast formation. Please see Figure 5b in the article [1].
The same clone (CGA7) is sold as Santa Cruz Biotechnology sc-53015; Sigma-Aldrich A7607; EMD Millipore MAB1522.
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