This is a knockout-validated antibody summary, based on the publication "Ascl2 inhibits myogenesis by antagonizing the transcriptional activity of myogenic regulatory factors", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2bκ

Company: Millipore

Antibody: Achaete Scute homolog 2

Catalog number: MAB4418

Summary: Mouse monoclonal IgG2bκ against a recombinant protein corresponding to human Achaete Scute homolog 2 (near the C-terminus). Reacts with human protein by western blot, immunocytochemistry and chromatin immunoprecipitation (ChIP).

Western blot | Immunohistochemistry

Muscles of WT, Ascl2-heterozygous (HE) and Ascl2-knockout (KO). WT: Muscle samples were washed with PBS and homogenized with radioimmune precipitation assay buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, and 0.1% SDS).

IHC: 5% goat serum, 2% BSA, 0.2% triton X-100, and 0.1% sodium azide in PBS.

WB: 1:1,000 dilution in 5% w/v nonfat dry milk.

IHC: 1:200 in blocking buffer overnight.

IHC: for 45 min at room temperature.

WB: enhanced chemiluminescence (ECL) Western blotting substrate (Pierce Biotechnology, Rockford, IL) and detected with FluoChem R imaging system (ProteinSimple, CA).

IHC: Leica DM 6000B fluorescence microscope.

The expression levels of Ascl2 in hindlimb muscles of Pax3+/+ (WT) and Pax3Cre/+/Ascl2flox/flox (KO) E17.5 mice as determined by western blot. Immunostaining of Pax7 (red), Ascl2 (green) and DAPI staining (blue) in back muscles of HE and KO mice at E17.5. Please see Figure 2a,c in the article [1].