Abcam goat polyclonal VDAC2 antibody ab37985 was examined in VDAC2−/− MEFs

DOI

http://dx.doi.org/10.13070/ko.en.6.1567

Date

2016-08-22

This is a knockout-validated antibody summary, based on the publication "Motifs of VDAC2 required for mitochondrial Bak import and tBid-induced apoptosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody / Gene

VDAC2, also called POR; outer mitochondrial membrane protein porin 2; voltage-dependent anion channel 2.

Catalog number

Catalog number is ab37985.

Summary

Goat polyclonal raised against a peptide corresponding to C terminal amino acids 283-294 of Human VDAC2. Reacts with Mouse, Rat, Human and has been used in western blot and immunofluorescence.

Abcam goat polyclonal VDAC2 antibody ab37985 was examined in VDAC2−/− MEFs figure 1

Figure 1. Western blot of VDAC2 (arrow) in wild type (WT), VDAC2 knockout (V2-/-) and rescued MEFs. A nonspecific band is also observed. From figure 1 [1].

Company

The supplier is Abcam.

Validation Method

Western blotting

Sample

VDAC2−/− MEFs

Detection

LI-COR Odyssey scanner

Notes

This antibody detects two bands at around 32–34 kDa in MEFs expressing V2; however, only the upper band is relevant because it is the one that is absent in the V2−/− MEFs and is restored in the genetically rescued cells.

References

Naghdi S, Varnai P, Hajnoczky G. Motifs of VDAC2 required for mitochondrial Bak import and tBid-induced apoptosis. Proc Natl Acad Sci U S A. 2015;112:E5590-9 pubmed publisher

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