This is a knockout-validated antibody summary, based on the publication "ATRX binds to atypical chromatin domains at the 3' exons of zinc finger genes to preserve H3K9me3 enrichment", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of ATRX in chromatin preparations from control (V2) and 2 CRISPR ATRX KO K562 cell lines. Amido Black staining of histones is shown as a loading control. From [1].

Rabbit polyclonal

Company: Sigma

Antibody: ATRX

Catalog number: HPA001906

Summary: Rabbit polyclonal against human ATRX isoform 5 recombinant protein. Reacts with human by immunoblotting and immunohistochemistry.

Western blot

Chromatin preparations from control and CRISPR ATRX KO K562 cell lines.

1/1,000 dilution.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.