ATM antibody | knockout validation

ATM antibody | knockout validation | Upstate 05-513

This is a knockout-validated antibody summary, based on the publication "Nocodazole-Induced Expression and Phosphorylation of Anillin and Other Mitotic Proteins Are Decreased in DNA-Dependent Protein Kinase Catalytic Subunit-Deficient Cells and Rescued by Inhibition of the Anaphase-Promoting Complex/Cyclosome with proTAME but Not Apcin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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ATM antibody | knockout validation | Upstate 05-513 figure 1

Figure 1. A549-CRISPR-control, A549–CRISPR–DNA-PKcs, and A549-CRISPR-ATM cells were treated with nocodazole for 6 or 16 h, and then extracts were run on SDS-PAGE and analyzed by western blotting. From [1].

Antibody information

Mouse monoclonal IgG

Company: Upstate

Antibody: ATM

Catalog number: 05-513

Summary: Mouse monoclonal IgG against full-length human ATM. Reacts with human by western blot.

Validation Method

Western blot

Sample

Control and ATM-KO A549 cells.

Detection

enhanced chemiluminescence (ECL) reagent and Fuji X-ray film

References

Douglas P, Ye R, Radhamani S, Cobban A, Jenkins N, Bartlett E, et al. Nocodazole-Induced Expression and Phosphorylation of Anillin and Other Mitotic Proteins Are Decreased in DNA-Dependent Protein Kinase Catalytic Subunit-Deficient Cells and Rescued by Inhibition of the Anaphase-Promoting Complex/Cyclosome with proTAME. Mol Cell Biol. 2020;40: pubmed publisher

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