This is a knockout-validated antibody summary, based on the publication "Loss of ATM accelerates pancreatic cancer formation and epithelial-mesenchymal transition" [2] (discussed below) and "CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism" [1] (see Figure 1 or figure 6b in the article). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot validation of CRISPR-Cas9 generated ATM knockout cell lines. From [1].

Mouse monoclonal IgG1

Company: Abcam

Antibody: ATM

Catalog number: ab78

Summary: Mouse monoclonal IgG1 against a fusion protein corresponding to amino acids 2577-3056 of human protein. Reacts with mouse, rat, human, and monkey. Suitable for western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

Mice pancreas from mice harbouring a floxed Atm (‘A'), p48Cre/+, and KrasG12D/+ (‘K'). Pancreas were homogenized in extraction buffer (4% SDS, 100 mM Tris-HCl) containing protease inhibitors and 1 mM phenylmethylsulfonyl fluoride (PMSF),

1:1000 dilution overnight at 4° C.

Immunoreactive bands were visualized using chemiluminescence (Thermo scientific, Waltham, MA, USA).

Please see Supplementary Figure 1 in the article [2].

The same clone (2C1) is sold as Novus Biologicals NB100-309; Santa Cruz Biotechnology sc-23921; LifeSpan Biosciences LS-C20116, LS-B1683; Abnova MAB12913; Invitrogen MA1-23152; Biorbyt orb197888; GeneTex GTX70103.