This is a knockout-validated antibody summary, based on the publication "Critical role of mitochondrial ubiquitination and the OPTN-ATG9A axis in mitophagy", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblots confirming KO of FIP200, ATG9A, and ATG5. I and II denote unmodified and lipidated LC3B, respectively. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: ATG9A

Catalog number: ab108338

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to a region within human ATG9A. Reacts with mouse, rat, and human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

WT and ATG9A-KO HeLa cells.

2% (wt/vol) skim milk/TBS with Tween 20 (TBS-T) for 20 min at room temperature.

Diluted in 1% (wt/vol) BSA/TBS-T for 2 h.

Goat anti-rabbit IgG horseradish peroxidase-linked (Jackson ImmunoResearch, catalog number 111-035-144) diluted in 2% (wt/vol) skim milk/TBS-T for 1hr.

Western Lightning Plus-ECL Kit (and ECL Prime Western Blotting Detection Reagents for PINK1) and an ImageQuant LAS 4000 imaging system (GE Healthcare).