This is a knockout-validated antibody summary, based on the publication "mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. SDS-PAGE and western blot analysis of whole cell lysates derived from HeLa (WT and ATG9a KO) treated with DMSO, rapamycin (20 uM), or rapamycin and bafilomycin A1 (1 uM) for four hours. Immunoblotting was performed with anti-ATG9a and anti-LC3. Tubulin served as loading control. From [1].

Rabbit monoclonal Ig

Company: Abcam

Antibody: ATG9A

Catalog number: ab108338

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to a region within human ATG9A. Reacts with mouse, rat, and human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

WT and ATG9a KO HeLa cells. Whole cell lysis was performed with radioimmunoprecipitation assay (RIPA) buffer (Thermo Fisher) supplemented with Halt Protease Inhibitor mixture EDTA-free (Thermo Fisher).

Odyssey blocking buffer in PBS (Li-Cor).

Secondary antibodies conjugated to DyLight 800 or 680 (Li-Cor).

Li-Cor Odyssey imaging system.