ATG7 | knockout validation | MBL PM039
DOI
//dx.doi.org/10.13070/ko.en.6.1777
Date
2016-11-07

This is a knockout-validated antibody summary, based on the publication "Divergent roles of BECN1 in LC3 lipidation and autophagosomal function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

ATG7 | knockout validation | MBL PM039 figure 1
Figure 1. Western blot for ATG7 protein in HeLa cells and ATG7 knockout cells. From [1].
Antibody information

Rabbit polyclonal

Company: MBL International Corporation

Antibody: ATG7

Catalog number: PM039

Summary: Raised against synthetic peptide from the C-terminal region of human ATG7C. It detects human ATG7. Supplier recommended for immunoprecipitation and Western blotting.

Validation Method

Western blot

Sample

HeLa cells and HeLa ATG7 -/- cells.

Primary incubation

Anti-ATG7 (MBL, PM039) – conditions not specified.

Secondary incubation

HRP-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch).

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. He R, Peng J, Yuan P, Xu F, Wei W. Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy. 2015;11:740-7 pubmed publisher