This is a knockout-validated antibody summary, based on the publication "Atg7 is required for acrosome biogenesis during spermatogenesis in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Atg7 in testes of control Atg7+/+ and TNAP-Cre Atg7−/− mice. GAPDH was used as loading control. Courtesy of the Authors [1].

Mouse monoclonal IgG1

Company: Sigma

Antibody: ATG7

Catalog number: SAB4200304

Summary: Mouse monoclonal IgG1 against a synthetic peptide corresponding to a sequence located near the N-terminus of human Atg7. Reacts with human, mouse, and rat. Suitable for western blot and immunoprecipitation.

Western blot

Testes of control and TNAP-Cre Atg7−/− mice. Tissue extracts were prepared using a Dounce homogenizer in cold RIPA buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate) supplemented with 1 mM phenylmethylsulfonyl fluoride and a protein inhibitor cocktail (Roche).

Alexa Fluor 680-conjugated goat anti-mouse secondary antibodies (Invitrogen, California, USA).

ODYSSEY Sa Infrared Imaging System (LI-COR Biosciences, USA).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.