ATG7 antibody | knockout validation | Abcam ab52472

This is a knockout-validated antibody summary, based on the publication "TMEM41B Is a Pan-flavivirus Host Factor", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

ATG7 antibody | knockout validation | Abcam ab52472 figure 1
Figure 1. Western blots for ATG5 and ATG7 in HAP1 WT cells and ATG5 and ATG7 KO clones. The expected sizes are indicated by labels on the right. β-actin is shown as loading control. The anti-ATG5 antibody also recognizes the ATG5/ATG12 conjugate with a higher molecular weight band. Also shown is the differently lipidated LC3 protein (LC3 I/II), indicative of functional autophagy. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: ATG7

Catalog number: ab52472

Summary: Rabbit monoclonal antibody against a synthetic peptide of human ATG7. Reacts with human by western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and flow cytometry (intra).

Validation Method

Western blot

Sample

Control and ATG7 KO HAP1 cells.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:5,000 dilution goat anti-rabbit IgG conjugated to HRP (ThermoFisher Scientific, 31462).

References
  1. Hoffmann H, Schneider W, Rozen Gagnon K, Miles L, Schuster F, Razooky B, et al. TMEM41B Is a Pan-flavivirus Host Factor. Cell. 2021;184:133-148.e20 pubmed publisher