ATG5 antibody | knockout validation | Sigma-Aldrich A2859

This is a knockout-validated antibody summary, based on the publication "3,4-Dimethoxychalcone induces autophagy through activation of the transcription factors TFE3 and TFEB", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

ATG5 antibody | knockout validation | Sigma-Aldrich A2859 figure 1
Figure 1. Immunoblot of the indicated proteins in WT and ATG5 knockout U2OS cells. From [1].
Antibody information

Mouse monoclonal IgG2a

Company: Sigma-Aldrich

Antibody: ATG5

Catalog number: A2859

Summary: Mouse monoclonal IgG2a against human Atg5. Reacts with human, rat, and mouse Atg5-Atg12 complex by immunoblotting (∼56kDa) and immunoprecipitation.

Validation Method

Western blot

Sample

WT and ATG5 knockout U2OS cells.

Primary incubation

1:1,000 dilution overnight at 4°C.

Secondary incubation

1:5,000 dilution horseradish peroxidase (HRP)‐conjugated secondary antibodies (Southern Biotech, Cambridge, UK).

Detection

ECL prime Western blotting detection reagent (Sigma‐Aldrich).

References
  1. Chen G, Xie W, Nah J, Sauvat A, Liu P, Pietrocola F, et al. 3,4-Dimethoxychalcone induces autophagy through activation of the transcription factors TFE3 and TFEB. EMBO Mol Med. 2019;11:e10469 pubmed publisher