ATG5 antibody | knockout validation | GeneTex GTX62601

This is a knockout-validated antibody summary, based on the publications "Mutations in the ubiquitin-binding domain of OPTN/optineurin interfere with autophagy-mediated degradation of misfolded proteins by a dominant-negative mechanism", as cited below [1], and "A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice" for western blot knockout validation (figure s3g) [2] s. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: GeneTex

Antibody: ATG5

Catalog number: GTX62601

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in human APG5L/ATG5. Reacts with human, mouse, and rat. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Wildtype and Atg5−/− MEF cell lines. Cells were lysed in RIPA buffer.

Secondary incubation

Peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch, 111-035-003).

Note

Western blot analysis of ATG5 in wildtype and Atg5-null (atg5−/−) MEF cells. GAPDH was used as loading control. Please see Figure 2e in the article [1].

References
  1. Shen W, Li H, Chen G, Chern Y, Tu P. Mutations in the ubiquitin-binding domain of OPTN/optineurin interfere with autophagy-mediated degradation of misfolded proteins by a dominant-negative mechanism. Autophagy. 2015;11:685-700 pubmed publisher
  2. Quach C, Song Y, Guo H, Li S, Maazi H, Fung M, et al. A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice. Nat Commun. 2019;10:5681 pubmed publisher