ATG3 antibody | knockout validation | MBL M133-3

This is a knockout-validated antibody summary, based on the publication "PTK2-mediated degradation of ATG3 impedes cancer cells susceptible to DNA damage treatment", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

ATG3 antibody | knockout validation | MBL M133-3 figure 1
Figure 1. Atg3 KO MEF cells were transfected with plasmids encoding MYC-his-ATG3 WT or ATG3Y203E. From [1].
Antibody information

Mouse monoclonal IgG2b,κ

Company: MBL

Antibody: ATG3

Catalog number: M133-3

Summary: Mouse monoclonal IgG2b,κ against recombinant Human Atg3. Reacts with human, mouse, rat and hamster by western blot, immunocytochemistry and immunoprecipitation.

Validation Method

Western blot

Sample

Atg3 KO MEFs with and without Atg3 recovery.

Detection

Phosphorimager.

References
  1. Ma K, Fu W, Tang M, Zhang C, Hou T, Li R, et al. PTK2-mediated degradation of ATG3 impedes cancer cells susceptible to DNA damage treatment. Autophagy. 2017;: pubmed publisher