This is a knockout-validated antibody summary, based on the publication "Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. From Figure 5 in [1]. Western blot of lysates of wild-type and ATG13 knockout mouse embryonic cells probed with anti-ATG13 antibody. Tubulin serves as a loading control.

Rabbit polyclonal

Company: Sigma-Aldrich

Antibody: ATG13

Catalog number: SAB4200100

Summary:

Rabbit polyclonal antibody raised against a KLH-conjugated synthetic peptide corresponding to residues 462-475 of human ATG13.

Supplier recommended for WB.

Reacts with human, mouse and rat ATG13.

Western blot

Lysates of wild-type and ATG13 -/-mouse embryonic fibroblasts.

Anti-ATG antibody diluted 1:1000.

HRP-conjugated anti-rabbit IgG (VWR) diluted 1:1000.

ECL reagent (GE Healthcare) with ImageJ densitometric quantitation.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.