This is a knockout-validated antibody summary, based on the publication "The eukaryotic initiation factor-2 kinase pathway facilitates differential GADD45a expression in response to environmental stress", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Santa Cruz Biotechnology
Antibody: ATF4 (CREB-2)
Catalog number: sc-200
Summary: Rabbit polyclonal antibody raised against an epitope within the C-terminal region of human ATF4 / CREB-2.
Supplier recommended for WB, IP, IF and elisa.
Reacts with mouse, rat, human, dog, pig and cow ATF4 / CREB-2.
Note: this antibody will be discontinued December 15, 2016.
Western blot
Lysates of murine embryonic fibroblasts (MEFs).
TBS containing 4% milk powder and 0.2% Tween 20.
Anti-ATF4 antibody.
HRP-conjugated secondary antibody.
Chemiluminescent substrate.
See figure 1 from [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- Jiang H, Jiang L, Wek R. The eukaryotic initiation factor-2 kinase pathway facilitates differential GADD45a expression in response to environmental stress. J Biol Chem. 2007;282:3755-65 pubmed
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.