This is a knockout-validated antibody summary, based on publications "ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases", as cited below [1], and "ATF3 downmodulates its new targets IFI6 and IFI27 to suppress the growth and migration of tongue squamous cell carcinoma cells" for western blot knockout validation (figure 2f, s5a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of IL-22-stimulated WT and ATF3-KO CMT93 cells for the expression of the indicated proteins. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: ATF3

Catalog number: ab207434

Summary: Rabbit monoclonal IgG against a recombinant full length protein within human ATF3 (aa 1 to the C-terminus). Reacts with mouse and human. Suitable for western blot, immunoprecipitation, and immunocytochemistry/immunofluorescence.

Western blot

WT and ATF3-KO CMT93 cells. Cells were lysed in 1x RIPA lysis buffer containing protease/ phosphatase inhibitors (Thermo Fisher).

5% skim dried milk in Tris buffered saline buffer (TBST: 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% Tween 20).

1:1,000 dilution overnight.

HRP conjugated secondary antibodies.

Chemiluminescence substrate (Omics Bio) and images were acquired using ImageQuant™ LAS 4,000 camera.