This is a knockout-validated antibody summary, based on the publication "Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: ASL

Catalog number: sc-374353

Summary: Mouse monoclonal IgG1 (kappa light chain) against an epitnope mapping between amino acids 1-29 at the N-terminus of human ASL. Recommended for detection of ASL of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including and canine and porcine.

Western blot | Immunohistochemistry

Liver from control and ASL-KO mice. WB: Tissue from 3 mice per group was homogenized in RIPA buffer (Sigma, catalog no. R0278-50 mL) containing proteases inhibitors (Roche, Basel, Switzerland, catalog no. 04693159001). IHC: Paraffin sections were treated with Antigen Retrieval Solution (Dako, Copenhagen, Denmark, catalog no. S236984) for 30 minutes.

WB: 3% bovine serum albumin.

IHC: 3% hydrogen peroxide for 5 minutes.

WB: 1:500 dilution.

IHC: 1:100 dilution at 4°C overnight.

WB: 1:20,000 dilution donkey anti-mouse immunoglobulin G/horseradish peroxidase (Jackson ImmunoResearch, West Grove, PA, catalog no.715-035-150).

IHC: M.O.M Vectastain and DAB detection kit (Vector Laboratories, Burlingame, CA, catalog no. PK-2200, and SK-4100, respectively) according to the manufacturer’s recommendations.

WB: Amersham ECL Western Blotting Detection Reagent (General Electric Healthcare Life Sciences, Marlborough, MA, catalog no. RPN2106).

Western blot for HPD and ASL 10 weeks after targeted editing in Hpd SLiK (Hpd- deleted) or Asl SLiK (Hpd- and Asl- deleted) mice. Serial sections of Asl SLiK liver lobes stained for either HPD ( E ) or ASL ( F ) in brown. Inset boxes are shown in higher magnification. Error bars indicate SEM. Scale bars are 50 μm. Please see Figure 1c,e,f in the article [1].