This is a knockout-validated antibody summary, based on the publication "ASK1 signalling regulates brown and beige adipocyte function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot against ASK1 in iBAT (n=9) from WT and ASK1-KO mice. Alpha-tubulin was used as loading control. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: ASK1

Catalog number: ab45178

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in the N-terminus of human ASK1. Reacts with mouse and human protein. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence and flow cytometry. Not suitable for immunoprecipitation.

Western blot

Interscapular brown adipose tissue (iBAT) from WT and ASK1-KO mice.

2% skim milk in TBS-T (50 mM Tris-HCl, 150 mM NaCl and 0.05% Tween20, pH 8.0).

1:10,000 dilution.

ECL system.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.