This is a knockout-validated antibody summary, based on the publication "T cell-intrinsic ASC critically promotes T(H)17-mediated experimental autoimmune encephalomyelitis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1κ

Company: Millipore

Antibody: ASC

Catalog number: 04-147

Summary: Mouse monoclonal IgG1κ against full length recombinant human ASC. Reacts with human and mouse by western blot and flow cytometry.

Western blot

T cells from Ascf/+Lck-Cre and Ascf/−Lck-Cre mice. Cells were lysed by lysis buffer (0.5% Triton X-100, 20 mM Hepes pH 7.4,150 mM NaCl, 12.5 mM β-glycerophosphate, 1.5 mM MgCl2, 10 mM NaF, 2 mM dithiothreitol, 1 mM sodium orthovanadate, 2 mM EGTA, 20 mM aprotinin, 1 mM phenylmethylsulfonyl fluoride).

1:1,000 dilution.

Western blot analysis of ASC expression in FACS-sorted naïve CD4+ T cells from control and Asc-KO mice. Please see Figure 1a in the article [1].