This is a knockout-validated antibody summary, based on the publication "ASC filament formation serves as a signal amplification mechanism for inflammasomes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. From Supplementary Figure S1 in From [1]. Western blot of lysates of wild-type and ASC knockout BMDMs probed with anti-ASC antibody. Actin serves as a loading control.

Rabbit polyclonal

Company: Adipogen Life Sciences

Antibody: ASC

Catalog number: AG-25B-006

Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to sequence in the N-terminal region of human ASC. Supplier recommended for WB, IP, ICC and IHC. Antibody is epitope affinity-purified. Reacts with mouse and human ASC.

Western blot

Lysates of wild-type and ASC -/- immortalised bone marrow derived macrophages (BMDMs).

TBS-T containing 5% milk powder.

Anti-ASC antibody diluted 1:1000 in blocking buffer.

HRP-conjugated secondary antibody diluted 1:5000 in blocking buffer.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.