This is a knockout-validated antibody summary, based on the publications "MLKL Activation Triggers NLRP3-Mediated Processing and Release of IL-1β Independently of Gasdermin-D", as cited below [1], and "Calpain drives pyroptotic vimentin cleavage, intermediate filament loss, and cell rupture that mediates immunostimulation" for western blot knockout validation (figure 1a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1

Company: MBL International

Antibody: ASC (TMS1)

Catalog number: D086-3

Summary: Mouse monoclonal IgG1 against Triton X-100 insoluble fraction of retinoic acid-treated HL60 cells. Reacts with human ASC protein by western blot and immunoprecipitation. It has been reported that it can be used in immunocytochemistry and immunohistochemistry.

Western blot

Control and ASC-KO THP-1 cells.

Goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, sc-2005).

ECL Western blotting substrate (Thermo Fisher Scientific, PI32209) and either standard autoradiography film (Pierce, Rockford, IL) or an electronic luminescence detection platform (ChemiDoc XRS+ system, 170-8265; Bio-Rad Laboratories, Hercules, CA).

Western blot of the indicated protein in CRISPR edited THP-1 cells. Actin was used as a load control. Please see Supplemental Figure 1b in the article [1].

RIPK3 (Novus Biologicals, NBP2-24588), NLRP3 (Adipogen, AG-20B-0014) are also KO validated (Fig S1).