This is a knockout-validated antibody summary, based on the publication "CARD8 is an inflammasome sensor for HIV-1 protease activity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: ASC/TMS1
Catalog number: 13833
Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the carboxy terminus of human ASC/TMS1 protein, isoform 1. Reacts with human by western blot, immunoprecipitation and immunohistochemistry (paraffin).
Western blot
Control and ASC KO THP-1 cells.
HRP-conjugated goat anti-rabbit IgG (#12-348, SigmaAldrich).
ChemiDoc Imaging System (Bio-Rad).
Figure: Knockouts of ASC in THP-1 cells were confirmed by immunoblotting. Please see Figure 4c in the article [1].
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
- gene