This is a knockout-validated antibody summary, based on the publication "An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. MCF10A cells were transduced with Lenti-iCas9-neo and LentiGuide carrying ARID2 sgRNA. Cells were then treated with or without 1 μg/ml doxycycline (DOX) for 72 h and analyzed by western blots. Tubulin was used as loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: ARID2

Catalog number: sc-166117

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1134-1316 mapping within an internal region of human ARID2. Recommended for detection of ARID2 of human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

MCF10A control and ARID2-KO cells.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.