This is a knockout-validated antibody summary, based on the publication "ARID1A loss in adult hepatocytes activates β-catenin-mediated erythropoietin transcription", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: ARID1A
Catalog number: ab182560
Summary: Rabbit monoclonal antibody against a recombinant fragment of human ARID1A . Reacts with human. It is predicted to work with mouse and rat. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), and flow cytometry (intra).
Western blot | Immunohistochemistry
WT and Arid1a KO mouse livers.
WB: 5% blocking reagent (Biorad 170–6404) in TBS/Tween 0.1% for 1 hr at RT.
WB 1:2,000 dilution overnight.
IHC: 1:1,000 dilution.
WB: 1:2,000 dilution HRP-conjugated secondary antibody (Cell Signaling, 7074).
IHC: 1:200 dilution biotinylated goat anti-rabbit (Vector lab, BA-1000).
WB: Clarity ECL substrate (Biorad 70–5061).
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